Monday, 19 March 2018 | 13:00 to 16:00

SC1: Optimizing Media for Culturing Cells

13:00 Introduction and Welcome

13:10 Modern Cell Culture Medium Development: An Introduction

Tim Beckmann, PhD, Director, Strategic Development & USP, Xell AG

Modern cell culture media formulations are a defined, but complex composition of often more than 50 raw materials. The quality of these individual components, their proportions as well as their interactions define the final media performance and robustness. This presentation will give an overview of critical points to consider when working with modern cell culture media and the design and production thereof. Finally, a few examples illustrate the potential of a targeted and rational medium development.

  • Chemically defined cell culture media and feed
  • Challenges in the context of cell culture media development
  • Cell culture media parameters and characterization

14:00 Chemical Aspects of Optimizing Cell Culture Media

Jochen B. Sieck, PhD, Head, Perfusion Systems Laboratory, Perfusion Systems and Cell Culture Media R&D, Merck KGaA

Optimization of mammalian cell culture media (CCM) is often limited by chemical aspects. For example, nutrients can only be increased to an (un)certain concentration, which depends on the solubility of the component itself but also various other components in the medium. The stability of CCM is affected by light, temperature and oxygen sensitive components. Variability in quality of single components can dramatically impact overall performance of the CCM. This talk will present a couple of case studies how these topics could be addressed successfully and give an outlook about what aspects remain to be studied further.

14:45 Refreshment Break

15:15 Optimizing Cell Culture Media: Advanced Methods to Characterize Media and Bioprocess

Frederic Girard, PhD, Managing Director, Eurofins Spinnovation Analytical

Cell culture process development and optimization are critical for the commercially viable production of recombinant proteins, cell therapy products and vaccines. Ideally, one wants to rapidly understand and monitor the factors that are most influential to cell-growth and production. Among these factors, the media and feeding strategy play a central role. We will present here innovative analytical methods applied to the development, characterization and troubleshooting of complex bioprocesses.

16:00 End of Optimizing Media for Culturing Cells Short Course

Instructor Biographies:

Tim_BeckmannTim Beckmann, PhD, Director, Strategic Development & USP, Xell AG

I did my PhD at Bielefeld University, cell culture technology group, working in the field of differences in specific productivity of CHO cells lines with a proteomic approach. The project combined different system biology approaches in cooperation with a pharma company from Switzerland. In parallel, I started working for the newly founded company Xell (under a different name at that time) which I joined full-time after graduation in 2012 as a scientific project manager. Today, I am part of the management team at Xell as Director of Strategic Development.

Jochen_SieckJochen B. Sieck, PhD, Head, Perfusion Systems Laboratory, Perfusion Systems and Cell Culture Media R&D, Merck KGaA

Jochen is a Biotechnologist by training. He got his PhD from ETH Zurich in Bioprocess Engineering. He worked in Novartis Bioprocess R&D before moving to become a lab head in cell culture media R&D in Merck KGaA, Darmstadt, Germany. He has more than 10 years of experience in bioreactor characterization, scale-up and scale-down of mammalian cell culture processes, perfusion and medium development.

Frederic_GirardFrederic Girard, PhD, Managing Director, Eurofins Spinnovation Analytical

Frederic obtained a PhD in Biophysical Chemistry at the CNRS in 2000 (Orleans, France). After a post-doc in Sweden (UmeƄ University), he founded the company Spinnovation Analytical in 2004 (Nijmegen, The Netherlands), dedicated to delivering advanced Analytical R&D. In 2014, Spinnovation operation moved to the former R&D site of MSD (Oss, The Netherlands), received GMP certification from the Dutch authorities and joined the group Sinensis Life Sciences, adding GMP services to its existing portfolio. In 2016, the group Sinensis was acquired by Eurofins and Spinnovation became Eurofins Centre of Excellence for NMR and Solid-State Characterization. Frederic Girard is currently Managing Director of Eurofins Spinnovation Analytical (Oss, The Netherlands).